Phosphotyrosine antibody

Catalog No. ABIN1177365

Cited in 3+ publications. The Mouse Monoclonal anti-Phosphotyrosine antibody (Clone PY20) (ABIN1177365) specifically detects Phosphotyrosine in ICS.

Quick Overview for Phosphotyrosine antibody (ABIN1177365)

Target: Phosphotyrosine

Host: Mouse

Clonality: Monoclonal

Conjugate: This Phosphotyrosine antibody is un-conjugated

Application: Intracellular Staining (ICS)

Clone: PY20

Product Details anti-Phosphotyrosine Antibody

Brand: BD Phosflow™

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography

Isotype: IgG2b

Application Details

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

References for anti-Phosphotyrosine antibody (ABIN1177365)

Arvidsson, Rupp, Nånberg, Downward, Rönnstrand, Wennström, Schlessinger, Heldin, Claesson-Welsh: "Tyr-716 in the platelet-derived growth factor beta-receptor kinase insert is involved in GRB2 binding and Ras activation." in: Molecular and cellular biology, Vol. 14, Issue 10, pp. 6715-26, (1994) (PubMed).

Fan, Mendelsohn, Masui, Kumar: "Regulation of epidermal growth factor receptor in NIH3T3/HER14 cells by antireceptor monoclonal antibodies." in: The Journal of biological chemistry, Vol. 268, Issue 28, pp. 21073-9, (1993) (PubMed).

Glenney, Zokas, Kamps: "Monoclonal antibodies to phosphotyrosine." in: Journal of immunological methods, Vol. 109, Issue 2, pp. 277-85, (1988) (PubMed).

Target Details for Phosphotyrosine

Target: Phosphotyrosine

Target Type: Amino Acid

Background: Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be autophosphorylated kinases or certain protein substrates that are regulated in oncogenesis or cell growth. Antibodies to phosphotyrosine provide one of the best tools for the detection and characterization of phosphotyrosine proteins.