NEK2 antibody (AA 287-299)

Catalog No. ABIN117981

The Rabbit Polyclonal anti-NEK2 antibody (ABIN117981) specifically detects NEK2 in EIA. The antibody is reactive with Human samples.

Quick Overview for NEK2 antibody (AA 287-299) (ABIN117981)

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK2 antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Product Details anti-NEK2 Antibody

Binding Specificity: AA 287-299

Sequence: G-R-Q-L-G-E-P-E-K-S-Q-D-S Remarks: Human NEK2 is a protein kinase involved in mitotic regulation at the G2-M transition and/or at meiosis.

Specificity: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Purification: Immunoaffinity chromatography.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 287-299 of Human NEK2 protein.

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA against the immunizingpeptide. Reactivity in other immunoassays is unknown. This product has been assayed byELISA against 0.1 μg of the immunizing peptide. A 1: 2,000 to 1: 8,000 dilution of theantibody is recommended for this assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.

Target Details for NEK2

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Alternative Name: NEK2

Background: NEK2 is closely related in its catalytic domain to the serine/threonine protein kinase NIMA of Aspergillus nidulans which is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the Nek2 protein is almost undetectable during G1 but accumulates progressively throughout S, reaching maximal levels in late G2. These observations demonstrate that NEK2 resembles Aspergillus NIMA, both in its catalytic domain, and cell cycle-dependent expression. Recombinant NEK2 is active as a serine/threonine-specific protein kinase and may undergo autophosphorylation. Both human NEK2 and fungal NIMA phosphorylate a similar, but not identical, set of proteins and synthetic peptides. NEK2 is shown to be expressed most abundantly in adult testis and there is increasing evidence that NEK2 is abnormally expressed in a wide variety of human cancers.Synonyms: HSPK 21, NLK1, NimA-related protein kinase 2, Serine/threonine-protein kinase Nek2

Gene ID: 4751

NCBI Accession: NP_001191111

UniProt: P51955

Pathways: M Phase