RPA2 antibody (pSer122)

Catalog No. ABIN118011

This anti-RPA2 antibody is a Rabbit Polyclonal antibody detecting RPA2 in WB and EIA. Suitable for Saccharomyces cerevisiae.

Quick Overview for RPA2 antibody (pSer122) (ABIN118011)

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPA2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-RPA2 Antibody

Binding Specificity: AA 115-129, pSer122

Sequence: S-S-G-K-G-Y-G-pS-Q-V-A-Q-Q-F-E Remarks: The RFA complex consists of a heterotrim er of RFA1, RFA2 and RFA3, 69, 36 and 13 kda chains respective ly. Yeast RFA2 is phosphoryl ated at the G1/S transition and is dephosphor ylated at mitosis.

Specificity: This affinity purified antibody is directed against yeast RFA2. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody is specific for phosphorylated pS122 of yeast RFA2. Reactivity with non-phosphorylated yeast RFA2 is minimal by ELISA.

Purification: Immunoaffinity chromatography.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 115-129 (of 273) of yeast DNA Replication Factor A (RFA2).

Application Details

Application Notes: This affinity-purified antibody was tested for use in western blotting against RFA2containing cell lysates and by ELISA against the immunizing peptide. Reactivity in otherimmunoassays is unknown. This product was assayed by western blotting against RFA2containing cell lysates. A 1: 500 to 1: 1000 dilution is recommended for this assay. Thisproduct was also assayed by ELISA against 0.1 μg of the phosphorylated form of theimmunizing peptide. A 1: 8000 to 1: 20000 dilution of the antibody is recommended for thisassay. Equivalent signal is seen against the non-phosphorylated form of the peptide whenthe antibody is diluted to 1: 300.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.54 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 2-8 °C.

Target Details for RPA2

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Alternative Name: RPA2

Background: Synonyms: REPA2, RF-A protein 2, RFA2, RP-A p32, RP-A p34, RPA32, RPA34, Replication factor A protein 2, Replication protein A, Replication protein A 32 kDa subunit, Replication protein A 34 kDa subunit

Gene ID: 6118

NCBI Accession: NP_002937

UniProt: P15927

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA