HIF3A antibody (AA 581-592)

Catalog No. ABIN118015

This Rabbit Polyclonal antibody specifically detects HIF3A in WB, IF, IHC (p) and EIA. It exhibits reactivity toward Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for HIF3A antibody (AA 581-592) (ABIN118015)

Target: HIF3A (Hypoxia Inducible Factor 3, alpha Subunit (HIF3A))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIF3A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-HIF3A Antibody

Binding Specificity: AA 581-592

Sequence: Mouse Hif3 ?, 662 residue, predicted MW 73.0 kDa 1 mdwdqdrsnt elrkeksrda arsrrsqete vlyqlahtlp fargvsahld kasimrltis 61 ylrmhrlcaa gewnqvekgg epldacylka legfvmvlta egdmaylsen vskhlglsql 121 elighsifdf ihpcdqeelq daltprpnls kkkleapter hfslrmkstl tsrgrtlnlk 181 aatwkvlhcs ghmraykppa qtspagsprs epplqclvli ceaiphpasl epplgrgafl 241 srhsldmkft ycderiaeva gyspddligc sayeyihald sdavsrsiht llskgqavtg 301 qyrflartgg ylwtqtqatv vsggrgpqse siicvhflis rveetgvvls leqteqhtrr 361 pprlsassqk gipgnsvdsp aprilaflhp palseaslaa dprrfcspdl rrlmapildg 421 pppaatpstp qatrrpqspl padlpdklav glenahrlst aqknktvetd ldiaqdsdtl 481 dlemlapyis mdddfqlnss eqlpkvhrrp prvarrprar sfhglsppip epsllprwgs 541 dprlncssps rgdrptaslm pgtrkralaq ssedkglell eikppkrspr lepgsfllpp 601 lslsfllqgr qllgnqqdpr aplvhshepl glapsllslc qheetvqprn hfppaaglgq 661 th

Specificity: This antibody recognizes Mouse HIF3A (581-592). A BLAST analysis was used to suggest cross reactivity with HIF3A protein from Mouse (100 % homology) and Rat (91 % homology). Reactivity against human HIF3A is not expected as nlz 75 % homology for the immunizing sequence is noted. No reactivity is expected against other forms of HIF proteins.

Cross-Reactivity (Details): Species reactivity (expected):Rat.
Species reactivity (tested):Mouse.

Purification: Immunoaffinity chromatography.

Immunogen: Synthetic peptide corresponding to aa 581-592 of mouse Hif3a

Application Details

Application Notes: ELISA (1/14,000-1/80,000). Westen blot (1/1,500-1/8,000), Expect a band approximately 72 kDa in size correspondingto Hif3 α protein by western blotting in the appropriate cell lysate or extract.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.2 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use

Storage: 4 °C

Storage Comment: Store vial at 2 - 8 °C prior to reconstitution. Following reconstitution the product is stable for one month at 2 - 8 °C as an undiluted liquid. . For extended storage add glycerol to 50 % and then aliquot contents and freeze at -20 °C or below.

References for anti-HIF3A antibody (ABIN118015)

Liu, DU, Huang, Luo, Wu, Chen: "[Mortality analyses for rungsten miners.]" in: Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases, Vol. 26, Issue 1, pp. 24-8, (2008) (PubMed).

Target Details for HIF3A

Target: HIF3A (Hypoxia Inducible Factor 3, alpha Subunit (HIF3A))

Alternative Name: HIF3A / HIF3 alpha

Background: One of the most important factors in the cellular response to hypoxia is hypoxia-inducible factor (HIF), which transcriptionally activates genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a heterodimer consisting of one of three subunits(HIF1-α, HIF2-α, or HIF3-α) bound to the aryl hydrocarbon receptor nuclear translocator(ARNT) that is also known as HIF1-β. HIF-α is a member of the basic helix-loop-helix (bHLH) superfamily, in which the HLH domain mediates subunit dimerization while the basic domain binds to DNA. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation, and cell survival, in fact, HIF3-α may be a marker for tumor growth and angiogenesis. Examples of HIF target genes include VEGF, glucose transporter 1 (GLUT1), and EPO. HIF binds to the hypoxia-responsive element, which contains the core recognition sequence 5'-TACGTG-3', in the cis-regulatory regions of hypoxia-inducible genes. Transcriptional activation by HIF is linked to its ability to recruit coactivator proteins such as CREB-binding protein (CBP), p300, steroid receptor coactivator-1, and translation initiation factor 2.Synonyms: BHLHE17, Basic-helix-loop-helix-PAS protein MOP7, Class E basic helix-loop-helix protein 17, HIF-3 alpha, Hypoxia-inducible factor 3 alpha, IPAS, Inhibitory PAS domain protein, MOP7, Member of PAS protein 7, PASD7

Gene ID: 64344

NCBI Accession: NP_690007

UniProt: Q9Y2N7

Pathways: Warburg Effect