Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
Specificity
This product was prepared from monospecific antiserum by a Delipidation and Defibrination. Assay by Immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Capsular Reactive Protein (CRP) [Human Serum]. Cross reactivity against Capsular Reactive Protein (CRP) from other tissues and species may occur but have not been specifically determined.
CRP
Reactivity: Rat
WB, IHC, ICC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore with 1.0 mL of deionized water (or equivalent).
Concentration
90.0 mg/mL (by Refractometry)
Buffer
0.01 M Sodium Phosphate, 0.11 M Sodium Chloride, pH 7.2 without preservatives.
Preservative
Without preservative
Handling Advice
Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Storage
4 °C/-20 °C
Storage Comment
Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below. For extended storage add glycerol to 50%.
The C-reactive protein (CRP) is a cyclic pentameric pentraxin family acute phase protein compound of five identical noncovalently bound nonglycosylated subunits (each subunit 24 kDa, physiologic CRP molecule 117,5 kDa). CRP is produced by the liver and its plasma levels rise dramatically during inflammatory processes occuring in the body. CRP is an initiator of classical complement cascade, binds to several nuclear components (chromatin, histones, etc.) and is also believed to play an important role in innate immunity. Patients with elevated basal levels of CRP are at increased risk for hypertension and cardiovascular disease.Synonyms: C Reactive Protein, PTX1, Pentraxin-related