ING5 antibody (AA 127-140)

Catalog No. ABIN118053

The Goat Polyclonal anti-ING5 antibody (ABIN118053) specifically detects ING5 in WB and EIA. The antibody is reactive with Human and Monkey samples.

Quick Overview for ING5 antibody (AA 127-140) (ABIN118053)

Target: ING5 (Inhibitor of Growth Family, Member 5 (ING5))

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This ING5 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-ING5 Antibody

Binding Specificity: AA 127-140

Sequence: Protein Sequence: Human p28 ING5 isoform 1, 240 aa, predicted MW 27.8 kDa: 1 matamylehy ldsienlpce lqrnfqlmre ldqrtedkka eidilaaeyi stvktlspdq 61 rverlqkiqn ayskckeysd dkvqlamqty emvdkhirrl dadlarfead lkdkmegsdf 121 essggrglkk grgqkekrgs rgrgrrtsee dtpkkkkhkg gseftdtils vhpsdvldmp 181 vdpneptycl chqvsygemi gcdnpdcpie wfhfacvdlt kpkgkwfcp rcvqekrkkk Protein Sequence: Human p28 ING5 isoform 2, 226 aa, predicted MW 25.9 kDa 1 matamylehy ldsienlpce lqrnfqlmre ldqrtedkka eidilaaeyi stvktlspdq 61 verlqkiqn ayskckeysd dkvqlamqty emvdkhirrl dadlarfead lkdkmegsdf 121 essggrglkk grgqkekrgs rgrgrrtsee dtpkkkkhkg gseftdtils vhpsdvldmp 181 vdpneptycl chqvsygemi gcdnpdcpie wfhfacvdlt tkpkgk

Specificity: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human p28 ING5 protein. The sequence is present in both isoforms 1 and 2 (see below). However, 100 % homology is on record for this protein from human and chimpanzee. Cross reactivity with p28 ING5 protein from frog, mouse, rat and chicken may not occur as sequence homology varies by one or more amino acids residues in this sequence as indicated by BLAST analysis. Reactivity with p28 ING5 proteins from other sources is not known.

Purification: Immunoaffinity chromatography.

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 127-140 of Human p28 ING5protein (Inhibitor of growth family, member 5). This sequence shows homology to isoforms 1 and 2 for ING5.

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA (1: 4,000 - 1: 16,000) and byWestern blot (1: 500-1: 1,000, Expect a band approximately 28 kDa or 26 kDa in sizecorresponding to variants of ING5 in the appropriate cell lysate or extract). Also suitable for Immunofluorescence microscopy.

Restrictions: For Research Use only

Handling

Concentration: 1.2 mg/mL (UVabs. 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.

Target Details for ING5

Target: ING5 (Inhibitor of Growth Family, Member 5 (ING5))

Alternative Name: ING5

Background: ING5 is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. ING5 is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. ING5 acts as a tumor suppressor and is a potent growth regulator in normal and in established cells. ING5 may have involvement in chromatin remodeling.Synonyms: Inhibitor of growth protein 5, p28ING5

Gene ID: 84289

NCBI Accession: NP_115705

UniProt: Q8WYH8

Pathways: Chromatin Binding