Albumin antibody (HRP)

Catalog No. ABIN118089

The HRP-conjugated Rabbit Polyclonal anti-Albumin antibody (ABIN118089) specifically detects Albumin in WB and EIA. The antibody is reactive with Human samples.

Quick Overview for Albumin antibody (HRP) (ABIN118089)

Target: Albumin (ALB)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Albumin antibody is conjugated to HRP

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Albumin Antibody (HRP)

Specificity: This reagent was purified from pooled rabbit sera obtained from rabbits immunized with Human Serum Albumin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, purified human serum albumin IgG, and human Serum.

Purification: Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification. The purified antibody was then conjugated to horseradish peroxidase and subsequently purified followed by exhaustive dialysis against the buffer stated below.

Immunogen: Human Serum Albumin.

Application Details

Application Notes: Suitable for Immunoblotting (western or dot blot), ELISA, Immunoperoxidase ElectronMicroscopy and Immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western Blot: 1/1,000-1/5,000. Immunohistochemistry: 1/500-1/2,500. Note: This product has been assayed against 1.0 μg of Human Albumin IgG in a standardcapture ELISA using TMB as a substrate for 30 minutes at room temperature. A working dilution of 1/2,000 to 1/10,000 of the reconstitution concentration is suggested

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL of deionized water (or equivalent).

Concentration: 1.0 mg/mL (before lyophilization by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Gentamicin Sulfate as preservative and Polyethylen Glycol (PEG-8000) as stabilizer.

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for Albumin

Target: Albumin (ALB)

Alternative Name: Albumin

Background: Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.Synonyms: ALB, BSA, HSA, Serum Albumin

Gene ID: 213

NCBI Accession: NP_000468

UniProt: P02768

Pathways: Lipid Metabolism