CD4 antibody

Catalog No. ABIN118115

The Rat Monoclonal anti-CD4 antibody has been validated for FACS and IHC (fro). It is suitable to detect CD4 in samples from Mouse.

Quick Overview for CD4 antibody (ABIN118115)

Target: CD4

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: YTS191-1

Product Details anti-CD4 Antibody

Specificity: This antibody recognises the CD4 cell surface antigen, expressed by a subset of T-lymphocytes. This clone exhibits depleting activity when used in vivo (Azide Free CD4 Antibody Cat.-No SM005LE is recommended for this purpose).

Purification: Affinity Chromatography on Protein G

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: Use 10 μL of 1/50-1/200 diluted antibody to label 10⁶ cells.
Immunohistochemistry on Frozen Sections: The epitope recognised by this antibody is reported to be sensitive to Formaldehyde Fixation and tissue processing. The use of Acetone fixation for frozen sections is recommended.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Gene ID: 12504

NCBI Accession: NP_038516

UniProt: P06332

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events