ITGA6 antibody (FITC)

Catalog No. ABIN118232

The Rat Monoclonal anti-ITGA6 antibody is suitable to detect ITGA6 in samples from Human, Mouse, Dog, Pig, Monkey and Sheep. It has been validated for FACS.

Quick Overview for ITGA6 antibody (FITC) (ABIN118232)

Target: ITGA6 (Integrin, alpha 6 (ITGA6))

Reactivity: Human, Mouse, Dog, Pig, Monkey, Sheep

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGA6 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: NKI-GoH3

Product Details anti-ITGA6 Antibody (FITC)

Specificity: This antibody recognises CD49F (VLA-6 alpha chain). Clone NKI-GoH3 reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukaemia cells (alpha-6 beta-1). In immunohistology the monoclonal antibody reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytotrophoblasts. VLA-6 is an important mediator of cell binding to laminin. Studies suggest that clone GoH3 specifically blocks the binding of cells to the E8 fragment of laminin.

Purification: Affinity chromatography on Protein G

Immunogen: BALB/c mouse mammary tumour cells. Spleen cells from immunised Sprague-Dawley rats were fused with cells of the SP2/0 mouse myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: Use 10 μL of neat antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ITGA6

Target: ITGA6 (Integrin, alpha 6 (ITGA6))

Alternative Name: CD49f / ITGA6

Background: CD49f is a 120kD cell surface glycoprotein that can form distinct complexes with CD29 (VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, which is expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex which is expressed on various human epithelial cells.Synonyms: CD49 antigen-like family member F, Integrin alpha-6, VLA-6, VLA6

Gene ID: 3655

NCBI Accession: NP_000201

UniProt: P23229

Pathways: CXCR4-mediated Signaling Events, Brown Fat Cell Differentiation, Integrin Complex