CD9 antibody (Biotin)

Catalog No. ABIN118500

The Biotin-conjugated Mouse Monoclonal anti-CD9 antibody (Clone MM2-57) (ABIN118500) specifically detects CD9 in FACS. The antibody is reactive with Human, Cow, Dog, Rabbit, Horse and Cat samples.

Quick Overview for CD9 antibody (Biotin) (ABIN118500)

Target: CD9

Reactivity: Human, Cow, Dog, Rabbit, Horse, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD9 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: MM2-57

Product Details anti-CD9 Antibody (Biotin)

Specificity: This antibody recognises CD9, a 24 kDa surface glycoprotein expressed by platelets, monocytes, some lymphocytes and endothelial cells.

Purification: Affinity Chromatography on Protein A

Immunogen: Human platelet membrane. Spleen cells from BALB/c mice immunised were fused with cells of the mouse SP2/0 myeloma line.

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: Use 10 μL of Neat-1/5 diluted antibody to label 10^6 cells or 100 μL whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: Contains 0.09 % Sodium Azide as preservative and 1 % Bovine Serum Albumin as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD9

Target: CD9

Alternative Name: CD9

Background: CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. It belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). It also regulates cell motility in a variety of cell lines, and appears to be an important regulator of Schwann cell behaviour in peripheral nerve.Synonyms: 5H9 antigen, Cell growth-inhibiting gene 2 protein, GIG2, Leukocyte antigen MIC3, MIC3, Motility-related protein, TSPAN29, Tetraspanin-29, p24

Gene ID: 928

NCBI Accession: NP_001760

UniProt: P21926

Pathways: Response to Water Deprivation, Cell-Cell Junction Organization