CD14 antibody (FITC)
Quick Overview for CD14 antibody (FITC) (ABIN118531)
Target
See all CD14 AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Purification
- Affinity Chromatography on Protein G
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Immunogen
- Human Thymocytes followed by peripheral blood mononuclear cells. Spleen cells from mice immunised BALB/c mice were fused with cells from the NS1-Ag4/1 mouse myeloma line.
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Isotype
- IgG2a
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Application Notes
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Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells or 100 μL whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 0.1 mg/mL
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Buffer
- PBS containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- CD14
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Alternative Name
- CD14
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Background
- The CD14 Molecule is found predominantly on monocytes and macrophages in flow cytometry, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein
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Gene ID
- 9606
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UniProt
- P08571
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Pathways
- TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
Target
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