CD27 antibody

Catalog No. ABIN118574

The Mouse Monoclonal anti-CD27 antibody is suitable to detect CD27 in samples from Human. It has been validated for FACS.

Quick Overview for CD27 antibody (ABIN118574)

Target: CD27 (CD27 Molecule (CD27))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD27 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: LT27

Product Details anti-CD27 Antibody

Specificity: The antibody precipitates a molecule of MW55 kDa (125 kDa in non-reduced conditions) designated as the CD27 antigen.

Purification: Ion exchange chromatography

Immunogen: Spleen cells from immunised mice were fused with cells of the P3. X 63-Ag8-653 mouse myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Western BLot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4

Preservative: Azide free

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody at 2 - 8 °C. DO NOT FREEZE!

Target Details for CD27

Target: CD27 (CD27 Molecule (CD27))

Alternative Name: CD27

Background: This antigen is expressed on activated T lymphocytes and belongs to a family of proteins associated with the nerve growth factor receptor. CD27 is expressed by thymocytes and peripheral T cells and also by EBV transformed B cell lines.Synonyms: CD27L receptor, T-cell activation antigen CD27, T14, TNFRSF7, Tumor necrosis factor receptor superfamily member 7

Gene ID: 939

NCBI Accession: NP_001233

UniProt: P26842

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints