CD35 antibody

Catalog No. ABIN118609

This Mouse Monoclonal antibody specifically detects CD35 in FACS, WB, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Baboon and Monkey.

Quick Overview for CD35 antibody (ABIN118609)

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reactivity: Human, Baboon, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD35 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: E11

Product Details anti-CD35 Antibody

Purification: Affinity Chromatography on Protein A

Immunogen: Human acute monocytic leukaemia cells. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 1/50 - 1/200, Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood. Immunohistochemistry on frozen sections: 1 μg/mL - 10 μg/mL. Immunohistochemistry on paraffin sections: 1 μg/mL - 10 μg/mL, This product requires antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Human Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Alternative Name: CD35 / C3BR

Background: CD35 is a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.Synonyms: C3b/C4b receptor, CR1, Complement receptor type 1

Gene ID: 9606

UniProt: P17927

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity