CD44 antibody (AA 3-10) (FITC)

Catalog No. ABIN118683

This anti-CD44 antibody is a Mouse Monoclonal antibody detecting CD44 in FACS. Suitable for Human.

Quick Overview for CD44 antibody (AA 3-10) (FITC) (ABIN118683)

Target: CD44

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD44 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: VFF-7

Product Details anti-CD44 Antibody (FITC)

Binding Specificity: AA 3-10

Specificity: This antibody specifically recognises an epitope encoded by exon v6 on the variant portion of Human CD44.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Immunogen: Glutathione S Transferase (GST) fusion protein corresponding to the variable domains (v3 to v10) of Human CD44. Spleen cells from immunised BALB/c micewere fused with cells of the P3X63Ag8.653 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Use 10 μL to label 10^6 Cells/100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: Tris buffered saline containing 0.02 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD44

Target: CD44

Alternative Name: CD44

Background: CD44 is the receptor for hyaluronic acid (HA). It mediates cell to cell and cell matrix interactions through its affinity for HA. Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. High protein heterogeneity due to several alternative splicing and post translational modification events. CD44R represents a heterogeneous group of CD44 variant isoforms expressed constitutively in epithelial cells and monocyte lineage cells and can be upregulated in activated leukocytes. CD44R, and in particular CD44v6 containing isoforms, may be involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to sites of inflammation. CD44v6 containing isoforms may be involved in tumor metastasis.Synonyms: CD44, CDw44, ECMR-III, Epican, Extracellular matrix receptor-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, LHR, MDU2, MDU3, MIC4, PGP-1, Phagocytic glycoprotein I

Gene ID: 960

NCBI Accession: NP_000601

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling