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ITGA2 antibody

This Mouse Monoclonal antibody specifically detects ITGA2 in FACS, WB, IP, IHC (p), IHC (fro) and EIA. It exhibits reactivity toward Human and Monkey.
Catalog No. ABIN118720

Quick Overview for ITGA2 antibody (ABIN118720)

Target

See all ITGA2 Antibodies
ITGA2 (Integrin alpha 2 (ITGA2))

Reactivity

  • 123
  • 56
  • 18
  • 9
  • 9
  • 9
  • 8
  • 6
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human, Monkey

Host

  • 80
  • 49
  • 19
  • 5
  • 5
Mouse

Clonality

  • 89
  • 72
Monoclonal

Conjugate

  • 75
  • 26
  • 17
  • 14
  • 7
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This ITGA2 antibody is un-conjugated

Application

  • 75
  • 72
  • 51
  • 38
  • 29
  • 27
  • 17
  • 11
  • 9
  • 6
  • 5
  • 2
  • 2
  • 2
Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone

16B4
  • Specificity

    This antibody is identified as capable of immunoprecipitating a non-reducible alpha subunit from I125 surface labelled cell line extract. It is confirmed as specific to the alpha 2 subunit by relative expression of antigen on various cell lines by FACS, and its recognition of affinity purified alpha 2 beta 1 in dot blots.

    Cross-Reactivity (Details)

    Species reactivity (tested):Human.

    Purification

    Affinity Chromatography on Protein G

    Immunogen

    Purified human beta1 preparation from HT1080 fibrosarcoma cell extract. Spleen cells from immunised BALB/c mice were fused with cells of the X63/Ag8.653 mouse myeloma cell line.

    Isotype

    IgG1
  • Application Notes

    Flow Cytometry: 30 μg/mL, Immunoprecipitation: 25-50 μg/mL. Immunohistochemistry on Frozen Sections: 30-40 μg/mL. ELISA: 10 μg/mL. Western Blot.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target

    ITGA2 (Integrin alpha 2 (ITGA2))

    Alternative Name

    CD49b / ITGA2

    Background

    CD49b, a member of the integrin family of extracellular matrix and cell adhesion molecules, is the integrin alpha-2 chain. CD49b associates noncovalently with CD29, the integrin beta-1 subunit, to form the heterodimer integrin a2b1 that binds laminin and collagen. It is expressed on B and T lymphocytes, NK cells, monocytes, platelets, fibroblasts and long-term cultured T cells. CD49b/CD29 mediates the Mg++ dependent adhesion of platelets to collagen. It may also be utilized by fibroblasts for the reorganization of the collagen matrix during would healing.Synonyms: CD49 antigen-like family member B, Collagen Receptor, GPIa, ITGA-2, Integrin alpha-2, Platelet membrane glycoprotein Ia, VLA-2 alpha, VLA2

    Gene ID

    3673

    NCBI Accession

    NP_002194

    UniProt

    P17301

    Pathways

    CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Integrin Complex
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