CD59 antibody

Catalog No. ABIN118747

This Mouse Monoclonal antibody specifically detects CD59 in WB, FACS, IP, IHC (p), IHC (fro) and EIA. It exhibits reactivity toward Human.

Quick Overview for CD59 antibody (ABIN118747)

Target: CD59

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD59 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: MEM-43

Product Details anti-CD59 Antibody

Specificity: This antibody reacts with a PI-linked glycoprotein, Mw 18-20 kDa found on all types of leucocytes including platelets. MEM-43 has been shown to stimulate NK activity.

Purification: Affinity chromatography on Protein A

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: 1/5 - 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. ELISA. Immunoprecipitation. Western Blot: The epitope recognised by MEM-43 is lost after reduction, therefore, non- reducing conditions are required for western blotting techniques. Immunohistochemistry on frozen and paraffin sections: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS

Preservative: Azide free

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Can be shipped at 2 - 8 °C.

Target Details for CD59

Target: CD59

Alternative Name: CD59

Background: The antigen is expressed on many haemapoietic and non-haemapoietic cells.Synonyms: 20 kDa homologous restriction factor, HRF-20, HRF20, MAC-IP, MAC-inhibitory protein, MACIF, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin

Gene ID: 966

NCBI Accession: NP_000602

UniProt: P13987

Pathways: Complement System