TCR alpha/beta antibody (Chain alpha)

Catalog No. ABIN118909

This Mouse Monoclonal antibody specifically detects TCR alpha/beta in IP and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for TCR alpha/beta antibody (Chain alpha) (ABIN118909)

Target: TCR alpha/beta (T Cell Receptor alpha/beta (TCR alpha/beta))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TCR alpha/beta antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 3A8

Product Details anti-TCR alpha/beta Antibody

Binding Specificity: Chain alpha

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein A

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry on frozen sections. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.09 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Target Details for TCR alpha/beta

Target: TCR alpha/beta (T Cell Receptor alpha/beta (TCR alpha/beta))

Alternative Name: T Cell Receptor (TCR) alpha/beta

Background: T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor beta locus. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. During T cell development, the beta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment, a V segment is then joined to the D-J gene. The C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase. Several V segments and one J segment of the beta locus are known to be incapable of encoding a protein and are considered pseudogenes. The beta locus also includes eight trypsinogen genes, three of which encode functional proteins and five of which are pseudogenes. Chromosomal abnormalities involving the T-cell receptor beta locus have been associated with T-cell lymphomas.Synonyms: T-Cell Receptor alpha, T-Cell Receptor alpha beta, T-Cell Receptor beta, TCRA, TCRB