CD235a/GYPA antibody

Catalog No. ABIN119043

The Rat Monoclonal anti-CD235a/GYPA antibody (Clone YTH89-1) (ABIN119043) specifically detects CD235a/GYPA in FACS, IHC (p), IHC (fro) and CyTox. The antibody is reactive with Human samples.

Quick Overview for CD235a/GYPA antibody (ABIN119043)

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This CD235a/GYPA antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox)

Clone: YTH89-1

Product Details anti-CD235a/GYPA Antibody

Specificity: This antibody recognises glycophorin A.

Purification: Affinity Chromatography on Protein G.

Immunogen: Human red blood cells followed by purified glycophorin A. Spleen cells from immunised DA rats were fused with cells of the Y3/Ag.1.2.3.

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: (1/10-1/25). Cytotoxic Assays. Immunhistochemistry on Frozen Sections (1/100-1/1000). Immunohistochemistry on Paraffin Sections (1/100-1/1000). This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended positive Control: Human hypophysis.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD235a/GYPA

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Alternative Name: CD235a / GYPA

Background: Glycophorin A is a major sialoglycoprotein of the human erythrocyte membrane.Synonyms: GPA, Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha

Gene ID: 2993

NCBI Accession: NP_002090

UniProt: P02724

Pathways: Maintenance of Protein Location