This antibody recognises the p85 subunit of PI-3 kinase.
Purification
Affinity chromatography on Protein G
Immunogen
Balculovirus expressed (Recombinant) Bovine P85 beta subunit of P13 kinase. Spleen cells from immunised mice were fused with cells of the NS1 mouse myeloma cell line.
Immunohistochemistry on frozen sections. Immunoprecipitation: 10 μg/mL. Western Blot: 1/1000 - 1/5000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.