CD19 antibody (FITC)
Quick Overview for CD19 antibody (FITC) (ABIN119121)
Target
See all CD19 AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Specificity
- This antibody recognises the CD19 cell surface antigen. It labels all B lymphocytes in peripheral blood and spleen but is negative on granulocytes, monocytes, platelets, T lymphocytes and erythrocytes. The antibody reacts with most B cell malignancies including chronic leukaemia and most lymphomas, but not multiple myeloma.
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Purification
- Affinity Chromatography on Protein G
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Isotype
- IgG1
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Application Notes
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Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 0.1 mg/mL
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Buffer
- PBS, pH 7.2, containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- CD19 (CD19 Molecule (CD19))
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Alternative Name
- CD19
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Background
- Human CD19 cell surface antigen is a 96 kD single chain glycoprotein on the surface of human B lineage cells, including pre-B cells. B cells can be detected in peripheral blood and tissues.Synonyms: B-cell marker, B-lymphocyte surface antigen B4, Differentiation antigen CD19, Leu-12
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Gene ID
- 930
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NCBI Accession
- NP_001171569
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UniProt
- P15391
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Pathways
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Target
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