CD40 Ligand antibody (FITC)

Catalog No. ABIN119174

Product Details anti-CD40 Ligand Antibody (FITC)

Target: CD40 Ligand (CD40LG)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD40 Ligand antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: This antibody recognizes the CD154 cell surface antigen. Clone MK13A4 has been reported to block binding of CD40 ligand to its receptor CD40. SM1555A is recommended for function studies.

Immunogen: hCG from Human pregnancy urine.

Clone: MK13A4

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: neat - 1:20, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Tris buffered saline containing 0.02 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD40 Ligand antibody (FITC) (ABIN119174)

Bose, Dasgupta, Tarafder, Bandyopadhyay: "Microwave-processed nanocrystalline hydroxyapatite: simultaneous enhancement of mechanical and biological properties." in: Acta biomaterialia, Vol. 6, Issue 9, pp. 3782-90, (2010) (PubMed).

Casati, Camisaschi, Novellino, Mazzocchi, Triebel, Rivoltini, Parmiani, Castelli: "Human lymphocyte activation gene-3 molecules expressed by activated T cells deliver costimulation signal for dendritic cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 6, pp. 3782-8, (2008) (PubMed).

Target Details for CD40 Ligand

Target: CD40 Ligand (CD40LG)

Alternative Name: CD154 / CD40L (CD40LG Products)

Synonyms: CD154 antibody, cd40l antibody, CD40L antibody, HIGM1 antibody, IGM antibody, IMD3 antibody, T-BAM antibody, TNFSF5 antibody, TRAP antibody, gp39 antibody, hCD40L antibody, CD40-L antibody, Cd40l antibody, Ly-62 antibody, Ly62 antibody, Tnfsf5 antibody, CD40 ligand antibody, TNF superfamily member 5 antibody, CD40LG antibody, cd40l antibody, Cd40lg antibody

Background: CD154 is a 32kD glycoprotein also known as CD40 ligand. CD154 is expressed on activated T lymphocytes, predominantly CD4+ve cells and also on some basophils and mast cells.Synonyms: CD40 ligand, CD40-L, CD40LG, GP39, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5

Gene ID: 959

NCBI Accession: NP_000065

UniProt: P29965

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints