CD34 antibody (FITC)

Catalog No. ABIN119261

The Rat Monoclonal anti-CD34 antibody has been validated for FACS. It is suitable to detect CD34 in samples from Mouse.

Quick Overview for CD34 antibody (FITC) (ABIN119261)

Target: CD34

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD34 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: MEC14-7

Product Details anti-CD34 Antibody (FITC)

Specificity: This antibody recognises the CD34 cell surface antigen. This antibody recognises a neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Clone MEC14.7 appears to recognise a subset of the stem cell population recognised by clone RAM34, and it is thought that this is due to differences in the epitope recognised by the two antibodies.

Purification: Affinity chromatography on Protein G

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: 1/5 - 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD34

Target: CD34

Alternative Name: CD34

Background: CD34 is expressed by endothelial cells and by haematopoietic stem cells.Synonyms: Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker

Gene ID: 12490

NCBI Accession: NP_598415

UniProt: Q64314