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His Tag antibody (HRP)

This anti-His Tag antibody is a Mouse Monoclonal antibody detecting His Tag in WB and EIA. Suitable for .

Western blot analysis of His-Ubiquitin recombinant protein probed with MOUSE ANTI HISTIDINE TAG:HRP (ABIN119352). (His Tag antibody (HRP))

1 image

Catalog No. ABIN119352

Quick Overview for His Tag antibody (HRP) (ABIN119352)

Target

See all His Tag Antibodies

His Tag

Reactivity

Please inquire

Host

106
65
11
10
9
8
2
1

Mouse

Clonality

Monoclonal

Conjugate

This His Tag antibody is conjugated to HRP

Application

167
116
40
33
31
30
28
16
15
13
13
13
7
6
5
4
4
3
3
3
2
2
1
1
1
1
1

Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone

AD1-1-10

Discover our top product His Tag Primary Antibody

Sequence

HHHHHH

Purification

Affinity Chromatography on Protein G

Immunogen

PAX6 transcription factor linked to histidine tag. Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.

Isotype

IgG1
anti-His Tag antibody
Reactivity: Human WB Host: Mouse Monoclonal 6AT18 unconjugated

anti-His Tag antibody
WB, ELISA, IHC, DB Host: Rabbit Polyclonal unconjugated

anti-His Tag antibody
WB, ELISA Host: Rabbit Polyclonal unconjugated

anti-His Tag antibody
WB, ELISA, IP, IF, FACS Host: Mouse Monoclonal 6G2A9 unconjugated

anti-His Tag antibody
WB, ELISA, IP Host: Mouse Monoclonal 33D10-D2-G8 unconjugated

anti-His Tag antibody
Reactivity: Human WB Host: Rabbit Polyclonal RB19960 unconjugated

anti-His Tag antibody
WB, ELISA, IP, DB, ISt Host: Mouse Monoclonal HIS-H8 unconjugated

anti-His Tag antibody (Cy3)
WB, ELISA, FACS, FM, DB, FLISA Host: Mouse Monoclonal 33D10-D2-G8 Cy3

anti-His Tag antibody (DyLight 800)
WB, ELISA, FM, DB, FLISA Host: Mouse Monoclonal 33D10-D2-G8 DyLight 800

anti-His Tag antibody (DyLight 649)
WB, ELISA, FM, DB, FLISA Host: Mouse Monoclonal 33D10-D2-G8 DyLight 649

Application Notes

ELISA: 1/20 - 1/1000. Western Blot: 1/1000 - 1/5000, In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

PBS, pH 7.4 containing 0.01 % Thiomersal as preservative and a HRP stabilizer

Storage

4 °C

Storage Comment

Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Target

His Tag

Alternative Name

6xHistidine Epitope Tag

Target Type

Tag

Background

6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.

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