beta-2 Microglobulin antibody

Catalog No. ABIN119386

This Mouse Monoclonal antibody specifically detects beta-2 Microglobulin in WB, FACS and IHC (fro). It exhibits reactivity toward Rat.

Quick Overview for beta-2 Microglobulin antibody (ABIN119386)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta-2 Microglobulin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: TLD-3H12B

Product Details anti-beta-2 Microglobulin Antibody

Specificity: This antibody recognises beta-2 microglobulin, expressed at the surface of most cells in association with MHC Class I molecules.

Purification: Affinity chromatography on Protein G

Immunogen: Rat microglial cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse MOM myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1/10 - 1/20, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for beta-2 Microglobulin

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Alternative Name: beta-2-Microglobulin

Background: Beta 2 Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.Synonyms: B2M, Beta 2 microglobulin, Beta-2 microglobulin

Gene ID: 24223

NCBI Accession: NP_036644

UniProt: P07151

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process