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SIRPA antibody (FITC)

Name: SIRPA antibody (FITC)
SKU: ABIN119468
Price: 780 USD
Availability: InStock

SIRPA Reactivity: Rat FACS Host: Mouse Monoclonal ED9 FITC

ABIN119468 staining of rat peritoneal macrophages (SIRPA antibody (FITC))

FACS

1 image

Catalog No. ABIN119468

Datasheet as PDF

Target See all SIRPA Antibodies

SIRPA (Signal-Regulatory Protein alpha (SIRPA))
Reactivity
74

39

34

10

9

3

3

3
Rat
Host
60

37

1

1
Mouse
Compatible Secondaries
Clonality
55

43

1
Monoclonal
Conjugate
54

10

6

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This SIRPA antibody is conjugated to FITC
Application
61

50

22

21

18

16

12

11

10

2

1

1

1
Flow Cytometry (FACS)

Specificity

This antibody recognises CD172a, also known as Signal regulatory protein (SIRP). ED9 binds to a different epitope on SIRP than OX41.

Purification

Affinity chromatography on Protein A

Immunogen

Spleen cell homogenate. Spleen cells from immunised mice were fused with cells of the Sp2/0 Ag-14 myeloma cell line.

Clone

ED9

Isotype

IgG1

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Application Notes

Flow cytometry: neat - 1/10, use 10 μL of the suggested working dilution to label 10^6 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

0.1 mg/mL

Buffer

Contains 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target

SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Alternative Name

CD172a / SIRPA (SIRPA Products)

Background

CD172a is expressed selectively by myeloid cells and by neurons.Synonyms: BIT, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, MFR, MYD1, Macrophage fusion receptor, MyD-1 antigen, PTPNS1, SHP substrate 1, SHPS-1, SHPS1, Signal-regulatory protein alpha-1, Sirp-alpha-2, Sirp-alpha-3, Tyrosine-protein phosphatase non-receptor type substrate 1, p84

Gene ID

25528

NCBI Accession

NP_037148

UniProt

P97710