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GZMB antibody (Granzyme B) Primary Antibody

GZMB Reactivity: Human IHC (p), WB Host: Mouse Monoclonal GB7 unconjugated
Catalog No. ABIN119563
$489.50
Plus shipping costs $45.00
0.1 mg
local_shipping Shipping to: United States
Available
  • Target
    GZMB
    Reactivity
    • 128
    • 46
    • 35
    • 12
    • 4
    • 3
    • 2
    • 1
    Human
    Host
    • 78
    • 50
    • 2
    Mouse
    Clonality
    • 80
    • 50
    Monoclonal
    Conjugate
    • 82
    • 14
    • 8
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GZMB antibody is un-conjugated
    Application
    • 72
    • 46
    • 40
    • 35
    • 22
    • 22
    • 19
    • 10
    • 8
    • 7
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Specificity
    This antibody recognises the serine protease Granzyme B, which is important in the induction of apoptosis in target cells by cytolytic lymphocytes (CTLs).
    Cross-Reactivity (Details)
    Species reactivity (tested):Human.
    Purification
    Affinity Chromatography on Protein A
    Immunogen
    Recombinant Granzyme B. Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line
    Clone
    GB7
    Isotype
    IgG2a
  • Application Notes
    Immunohistochemistry on Paraffin Sections (1/50-1/200): Requires antigen retrieval using heat treatment with sodium citrate buffer pH 6.0. Western blot.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    TRIS buffered saline, pH 8.0, 0.01 % Thiomersal
    Preservative
    Thimerosal (Merthiolate)
    Precaution of Use
    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    GZMB
    Abstract
    GZMB Antibody Abstract
    Synonyms
    CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT, granzyme B, GZMB
    Background
    Granzyme A and Granzyme B are serine proteases that mediate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Both granzyme A and granzyme B are synthesized as inactive proenzymes, and they are stored within cytolytic granules and released by effector cells during degranulation. In activated CTLs, granzyme A and granzyme B are processed and activated by cathepsin C, and they then function to induce apoptosis by two distinct pathways. Granzyme B proteolytically cleaves and activates members of the caspase family of cysteine proteases, including caspase-3, caspase-6, caspase-7 and caspase-9. When cleaved, these caspases assemble into active holoenzymes that then mediate apoptosis through a defined proteolytic cascade involving nuclear lamins and PARP (poly ADP ribose polymerase). Granzyme A mediates the activation of apoptosis by inducing single-strand DNA breaks, membrane perturbation and nuclear condensations in an alternative pathway that is independent from caspase activation or the caspase proteolytic cascade.Synonyms: CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Cathepsin G-like 1, Cytotoxic T-lymphocyte proteinase 2, Fragmentin-2, GRB, Granzyme-2, Lymphocyte protease, SECT, T-cell serine protease 1-3E
    Gene ID
    3002
    NCBI Accession
    NP_004122
    UniProt
    P10144
    Pathways
    Apoptosis, Caspase Cascade in Apoptosis
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