GZMB antibody

Catalog No. ABIN1302451

Product Details anti-GZMB Antibody

Target: GZMB (Granzyme B (GZMB))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This GZMB antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody CLB-GB11 recognizes granzyme B, a 31 kDa serine protease expressed intracellularly in activated Tc cells and NK cells.

Cross-Reactivity (Details): Human, Non-Human Primates

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human NK cell line YT-INDY-derived granzyme B

Clone: CLB-GB11

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-GZMB antibody (ABIN1302451)

Rissoan, Duhen, Bridon, Bendriss-Vermare, Péronne, de Saint Vis, Brière, Bates: "Subtractive hybridization reveals the expression of immunoglobulin-like transcript 7, Eph-B1, granzyme B, and 3 novel transcripts in human plasmacytoid dendritic cells." in: Blood, Vol. 100, Issue 9, pp. 3295-303, (2002) (PubMed).

Tak, Spaeny-Dekking, Kraan, Breedveld, Froelich, Hack: "The levels of soluble granzyme A and B are elevated in plasma and synovial fluid of patients with rheumatoid arthritis (RA)." in: Clinical and experimental immunology, Vol. 116, Issue 2, pp. 366-70, (1999) (PubMed).

Spaeny-Dekking, Hanna, Wolbink, Wever, Kummer, Kummer, Swaak, Middeldorp, Huisman, Froelich, Hack: "Extracellular granzymes A and B in humans: detection of native species during CTL responses in vitro and in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 7, pp. 3610-6, (1998) (PubMed).

Target Details for GZMB

Target: GZMB (Granzyme B (GZMB))

Alternative Name: Granzyme B (GZMB Products)

Synonyms: CCPI antibody, CGL-1 antibody, CGL1 antibody, CSP-B antibody, CSPB antibody, CTLA1 antibody, CTSGL1 antibody, HLP antibody, SECT antibody, granzyme B antibody, GZMB antibody

Background: Granzyme B,Granzyme B is a serine protease that is expressed in cytoplasmic granules of cytotoxic T lymphocytes and NK cells. Vectorial secretion of perforin and granzymes is responsible for their granule-mediated cytotoxicity. Granzyme B plays a pivotal role in the induction of apoptosis in the target cells by activation of caspases. Moreover, granzyme B was reported to cleave directly alpha-tubulin, leading to perturbation of microtubule networks during the induced cell death.,GZMB, HLP, CTLA1, SECT

Gene ID: 3002

UniProt: P10144

Pathways: Apoptosis, Caspase Cascade in Apoptosis