Aquaporin 1 antibody (AA 198-208)

Catalog No. ABIN119569

This Mouse Monoclonal antibody specifically detects Aquaporin 1 in WB, IP, IHC (p), IHC (fro) and EIA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Aquaporin 1 antibody (AA 198-208) (ABIN119569)

Target: Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Aquaporin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 7D11

Product Details anti-Aquaporin 1 Antibody

Binding Specificity: AA 198-208

Specificity: This antibody recognises an epitope within an extracellular region of the water-specific channel aquaporin 1 (also known as AQP1 or CHIP-28)

Purification: Affinity Chromatography on Protein G

Immunogen: Synthetic peptide corresponding to amino acids 198-208 of Rat Aquaporin 1. Spleen cells from immunised Balb/c mice were fused with the cells of Sp-2/0 Ag14 mouse myeloma cell line.

Isotype: IgG2b

Application Details

Application Notes: ELISA: 1/1000 - 1/10000. Immunoprecipitation: 1/1000 - 1/10000. Western Blot: 1/1000 - 1/2000. Immunohistochemistry on frozen and paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Aquaporin 1

Target: Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Alternative Name: Aquaporin-1

Background: Aquaporin 1 is a 28kD integral membrane protein which was originally identified in red blood cells and renal proximal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. The glycosylated forms of AQP1 range between 40-60kD in mass.Synonyms: AQP-1, AQP1, Aquaporin-CHIP, CHIP28, Urine water channel, Water channel protein for red blood cells and kidney proximal tubule

Gene ID: 358

NCBI Accession: NP_932766

UniProt: P29972

Pathways: Hormone Transport