CTLA4 antibody

Catalog No. ABIN119631

The Mouse Monoclonal anti-CTLA4 antibody (Clone WKH203) (ABIN119631) specifically detects CTLA4 in WB, FACS, IHC (fro) and EIA. The antibody is reactive with Rat samples.

Quick Overview for CTLA4 antibody (ABIN119631)

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTLA4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: WKH203

Product Details anti-CTLA4 Antibody

Specificity: This antibody recognises CD152, also known as cytotoxic-T-lymphocyte antigen-4 (CTLA-4).

Purification: Affinity chromatography on Protein G

Immunogen: Purified rCTLA-4hIg fusion protein. Spleen cells from immunised Balb/c were fused with cells of the X63-Ag8-653 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1:10 - 1:20, use 10 μL to label 10^6 cells in 100 μL, membrane permeabilisation is required for this application. ELISA. Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CTLA4

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Alternative Name: CD152 / CTLA4

Background: CD152 is similar in structure to CD28 and also binds ligands CD80 and CD86. CD152 is expressed by activated T-lymphocytes. Studies suggest that CD152 is also expressed by regulatory T-lymphocytes.Synonyms: CTLA-4, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4

Gene ID: 63835

NCBI Accession: NP_113862

UniProt: Q62859

Pathways: Cancer Immune Checkpoints