CD13 antibody

Catalog No. ABIN119653

This Rat Monoclonal antibody specifically detects CD13 in FACS and IHC (fro). It exhibits reactivity toward Mouse.

Quick Overview for CD13 antibody (ABIN119653)

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD13 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: R3-63

Product Details anti-CD13 Antibody

Specificity: This antibody recognises aminopeptidase N (APN), a cell surface protein homologous with human CD13. Clone R3-63 has been reported to block mouse APN enzyme activity. We recommend the use of SM1856A for this purpose.

Purification: Affinity chromatography on Protein G

Immunogen: Mouse intestinal APN. Spleen cells from immunised mice were fused with cells of the IR983F rat myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: 1/50 - 1/100, use 10 μL of the suggested working dilution to label 106 cells. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD13

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Alternative Name: CD13

Background: In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kD subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells.Synonyms: ANPEP, APN, Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, PEPN, gp150

Gene ID: 16790

NCBI Accession: NP_032512

UniProt: P97449

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones