CD177 antibody

Catalog No. ABIN119768

The Mouse Monoclonal anti-CD177 antibody is suitable to detect CD177 in samples from Human and Primate. It has been validated for WB, FACS and IP.

Quick Overview for CD177 antibody (ABIN119768)

Target: CD177 (CD177 Molecule (CD177))

Reactivity: Human, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD177 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: MEM-166

Product Details anti-CD177 Antibody

Specificity: This antibody reacts with CD177 (Neutrophil specific antigen 1), a 60 kDa GPI-linked cell surface glycoprotein of uPAR family, expressed on granulocytes and in bone marrow early erythroblasts, megakaryocytes, promyelocytes and myelocytes.

Cross-Reactivity (Details): Species reactivity (tested):Human, Non-Human Primates.

Purification: Affinity Chromatography on Protein A

Immunogen: Human Granulocytes

Isotype: IgG1

Application Details

Application Notes: Flow cytometry. Immunoprecipitation. Western Blot under non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated thawing and freezing.

Storage: 4 °C/-20 °C

Storage Comment: Store the original vial at 2-8 °C, stock solution or aliquots thereof at -20 °C.

Target Details for CD177

Target: CD177 (CD177 Molecule (CD177))

Alternative Name: CD177

Background: CD177 (NB1/HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31(PECAM-1). CD177 participates in neutrophil transmigration and seems to be also a pro-proliferative molecule. The antibodies against CD177 can be involved in neonatal alloimmune neutropenia (NAN).Synonyms: HNA-2a, HNA2a, Human neutrophil alloantigen 2a, NB1, NB1 glycoprotein, PRV1, Polycythemia rubra vera protein 1

Gene ID: 57126

NCBI Accession: NP_065139

UniProt: Q8N6Q3