S100A8/A9 Complex (Calprotectin) antibody (FITC)

Catalog No. ABIN119772

This anti-S100A8/A9 Complex (Calprotectin) antibody is a Mouse Monoclonal antibody detecting S100A8/A9 Complex (Calprotectin) in FACS. Suitable for Human, Pig, Cow, Cat, Dog, Guinea Pig, Monkey, Rabbit, Rat and Horse.

Quick Overview for S100A8/A9 Complex (Calprotectin) antibody (FITC) (ABIN119772)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Reactivity: Human, Pig, Cow, Cat, Dog, Guinea Pig, Monkey, Rabbit, Rat, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A8/A9 Complex (Calprotectin) antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: MAC387

Product Details anti-S100A8/A9 Complex (Calprotectin) Antibody (FITC)

Specificity: This antibody recognises the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 12 kDa beta chain. The antigen recognized by this anti Macrophages antibody is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia.

Purification: Affinity Chromatography on Protein G.

Immunogen: Human Monocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for S100A8/A9 Complex (Calprotectin)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Alternative Name: MRP8 / MRP14

Background: Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becomming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognised as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).Synonyms: MRP8/14, S100A8, S100A8/A9, S100A9

UniProt: P06702

Pathways: S100 Proteins