IL-2 antibody (FITC)

Catalog No. ABIN119946

This anti-IL-2 antibody is a Rat Monoclonal antibody detecting IL-2 in FACS. Suitable for Human and Monkey.

Quick Overview for IL-2 antibody (FITC) (ABIN119946)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Human, Monkey

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-2 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: MQ1-17H12

Product Details anti-IL-2 Antibody (FITC)

Purification: Affinity chromatography on Protein G

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry (neat - 1/10): Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Membrane permeabilisation is required for this application.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: Interleukin-2 / IL2

Background: Interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells.Synonyms: IL-2, T-cell growth factor, TCGF

Gene ID: 9606

UniProt: P60568

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation