ITGA5 antibody (FITC)

Catalog No. ABIN119996

The Rat Monoclonal anti-ITGA5 antibody is suitable to detect ITGA5 in samples from Mouse. It has been validated for FACS.

Quick Overview for ITGA5 antibody (FITC) (ABIN119996)

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGA5 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: MFR5 (5H10)

Product Details anti-ITGA5 Antibody (FITC)

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Affinity Chromatography on Protein G

Immunogen: Mouse MC/9 cell line. Remarks: Spleen cells from immunised Lewis rats were fused with cells of the Murine P3X63Ag8.653

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ITGA5

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Alternative Name: CD49e / ITGA5

Background: Integrin alpha 5 belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post translational cleavage in the extracellular domain to yield disulfide linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell surface mediated signalling. It is expressed on thymocytes, activated T cells, mast cells, and a variety of mouse cell lines.Synonyms: CD49 antigen-like family member E, FNRA, Fibronectin receptor subunit alpha, Integrin alpha-5, Integrin alpha-F, VLA-5, VLA5

Gene ID: 10090

UniProt: P11688

Pathways: Integrin Complex