TUBA1B antibody

Catalog No. ABIN120144

The Rat Monoclonal anti-TUBA1B antibody (Clone YL1-2) (ABIN120144) specifically detects TUBA1B in WB, IF, IHC (fro), IP, EIA and RIA. The antibody is reactive with Avian, Mammalian and Saccharomyces cerevisiae samples.

Quick Overview for TUBA1B antibody (ABIN120144)

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Reactivity: Avian, Mammalian, Saccharomyces cerevisiae

Host: Rat

Clonality: Monoclonal

Conjugate: This TUBA1B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA)

Clone: YL1-2

Product Details anti-TUBA1B Antibody

Specificity: SM2202P recognises the alpha subunit of Tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin) (2). The epitope recognised by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized Actin (5).

Purification: Affinity Chromatography on Protein G

Immunogen: Yeast Tubulin. Spleen cells from immunised LOU rats were fused with cells of the Y3.Ag.1.2.3 rat myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry on frozen sections. ELISA (1/100-1/1000, routinely tested in ELISA on Tubulin) (3). Immunoprecipitation. Radioimmunoassay. Clone YL1/2 has also been reported to work in western blotting (3).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline pH 7.4, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for TUBA1B

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Alternative Name: alpha Tubulin / TUBA1B

Background: Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.Synonyms: Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain

Gene ID: 10376

NCBI Accession: NP_006073

UniProt: P68363

Pathways: Microtubule Dynamics, M Phase