MHC Class I RT1.Aa alpha-Chain (RT1.AA) antibody

Catalog No. ABIN120149

The Mouse Monoclonal anti-MHC Class I RT1.Aa alpha-Chain antibody is suitable to detect MHC Class I RT1.Aa alpha-Chain in samples from Rat. It has been validated for FACS and IHC (fro).

Quick Overview for MHC Class I RT1.Aa alpha-Chain (RT1.AA) antibody (ABIN120149)

Target: MHC Class I RT1.Aa alpha-Chain (RT1.AA)

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: F16-4-4

Product Details

Purification: Affinity chromatography on Protein G

Immunogen: Partially purified rat MHC antigens. Spleen cells of immunised BALB/c mice were fused with cells from the NS-1 mouse myeloma line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry (1/100-1/200). Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: MHC Class I RT1.Aa alpha-Chain (RT1.AA)

Alternative Name: MHC Class I (RT1Aa)

Background: MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.Synonyms: RT1 class I histocompatibility antigen AA alpha chain

Gene ID: 10116

UniProt: P16391