LAMP2 antibody

Catalog No. ABIN120164

The Rat Monoclonal anti-LAMP2 antibody is suitable to detect LAMP2 in samples from Mouse. It has been validated for FACS, IP and IHC (fro).

Quick Overview for LAMP2 antibody (ABIN120164)

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This LAMP2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: M3-84

Product Details anti-LAMP2 Antibody

Specificity: This antibody recognises CD107b, also known as MAC-3 and LAMP-2.

Cross-Reactivity (Details): Species reactivity (tested):Mouse

Purification: Affinity chromatography on Protein G

Immunogen: Glycoproteins purified from mouse peritoneal macrophage membranes. Spleen cells from immunised Lewis rats were fused with cells of the mouse P3-NSI/1-Ag4-1 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL,Results may be enhanced using permeabilisation. Immunoprecipitation. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for LAMP2

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Alternative Name: CD107b / LAMP2

Background: CD107b is a transmembrane glycoprotein that is associated with lysosomal membranes and is primarily expressed on mononuclear phagocytes. Expression of CD107b does vary between cell populations and the molecular weight of CD107b can vary between 92-120kD. Studies suggest that CD107b may be involved in leucocyte adhesion.Synonyms: LAMP-2, LAMP-2C, LAMPB, Lysosome-associated membrane glycoprotein 2

Gene ID: 16784

NCBI Accession: NP_001017959

UniProt: P17047

Pathways: Autophagy