IL2 Receptor beta antibody

Catalog No. ABIN120202

Product Details anti-IL2 Receptor beta Antibody

Target: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL2 Receptor beta antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Specificity: This antibody recognises the CD122 cell surface glycoprotein. Clone TM-beta 1 is reported to block IL-2 high affinity binding to the IL-2R beta receptor. We recommend the use of SM2224LE for this purpose.

Purification: Affinity Chromatography on Protein G

Immunogen: Cells from murine IL-2RB+ transformant TART-mBeta. Lymph node (cells from immunised Sprague Dawley rats were fused with cells of the mouse PA1 myeloma cell line.

Clone: TM-BETA1

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: Use 10 μL of 1/50-1/100 diluted antibody to label 10^6 cells in 100 μL. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IL2 Receptor beta

Target: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Alternative Name: CD122 / IL2RB (IL2RB Products)

Background: CD122 is a 75-85kD molecule also known as interleukin-2 receptor (IL-2R) beta chain. In mouse, CD122 is primarily expressed on natural killer (NK) cells and at lower levels on lymphocyte subsets, monocytes and macrophages. CD122 can associate with the IL-2R alpha chain (CD25) and CD132 to form a functional high affinity receptor complex that binds IL-2. CD132/CD122 heterodimers can also be formed which bind IL-2 with intermediate affinity. IL-2 binding to the IL-2R is important in the generation and differentiation of T lymphocytes and NK cells.Synonyms: IL-2 receptor subunit beta, IL2 Receptor beta, Interleukin-2 receptor subunit beta, P70-75

Gene ID: 16185

NCBI Accession: NP_032394

UniProt: P16297

Pathways: JAK-STAT Signaling, Growth Factor Binding