NCR1 antibody (PE)

Catalog No. ABIN120215

The Mouse Monoclonal anti-NCR1 antibody has been validated for FACS. It is suitable to detect NCR1 in samples from Cow.

Quick Overview for NCR1 antibody (PE) (ABIN120215)

Target: NCR1 (Natural Cytotoxicity Triggering Receptor 1 (NCR1))

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This NCR1 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: AKS1

Product Details anti-NCR1 Antibody (PE)

Specificity: This antibody is specific for CD335, also known as NKp46 and Natural cytotoxicity triggering receptor 1. Clone AKS1 is reported to activate lysis of FcgammaR-expressing cell line P815, by IL-2 activated NKp46+ cells.

Purification: Affinity Chromatography on Protein G.

Immunogen: Fusion protein consisting of the extracellular region of bovine CD335. Spleen cells from immunised Balb/c mice were fused with cells of the NS-0 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: neat- 1/5, use 10 μL of the suggested working dilution to label 106 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL distilled water

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Lyophilized and reconstituted antibody ist stable at 2 - 8 °C for up to one month or at -20 °C for longer.

Target Details for NCR1

Target: NCR1 (Natural Cytotoxicity Triggering Receptor 1 (NCR1))

Alternative Name: CD335 / NKp46

Background: CD335 is a type I transmembrane protein, with two extracellular C2-type immunoglobulin-like domains, which functions as an activating receptor. CD335 is primarily expressed by a subset natural killer (NK) cells. No expression of CD335 has been detected on B cells, T cells, monocytes or granulocytes.Synonyms: LY94, NCR1, NK cell-activating receptor, NKp46, Natural cytotoxicity triggering receptor, Natural killer cell p46-related protei

Gene ID: 369024

NCBI Accession: NP_899209

UniProt: Q863H2

Pathways: Regulation of Leukocyte Mediated Immunity