MADCAM1 antibody

Catalog No. ABIN120237

The Mouse Monoclonal anti-MADCAM1 antibody (Clone 314G8) (ABIN120237) specifically detects MADCAM1 in WB, FACS, IHC (p), Func and EIA. The antibody is reactive with Human samples.

Quick Overview for MADCAM1 antibody (ABIN120237)

Target: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MADCAM1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Enzyme Immunoassay (EIA)

Clone: 314G8

Product Details anti-MADCAM1 Antibody

Specificity: This antibody recognises Human mucosal addressin cell adhesion molecule 1 (MAdCAM-1). Clone 314G8 does not recognise MAdCAM-1 in the gut lamina propria. Clone 314G8 reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognised by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7.

Purification: Affinity Chromatography on Protein G

Endotoxin Level: Low Endotoxin

Immunogen: Recombinant soluble MAdCAM-1-fc fusion protein. Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: ELISA. Western Blot: this antibody detects a band of approximately 60 kDa. Functional Assays. Flow Cytometry: 1/10 - 1/100, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on paraffin embedded sections: This product does not require protein digestion pre-treatment of paraffin embedded sections prior to staining, This product does not require antigen retrieval prior to staining of paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 without preservatives or stabilizers

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for MADCAM1

Target: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Alternative Name: MAdCAM1

Background: MAdCAM-1 is a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyers patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria.Synonyms: MAdCAM-1, Mucosal addressin cell adhesion molecule 1, hMAdCAM-1

Gene ID: 8174

NCBI Accession: NP_570116

UniProt: Q13477