2B4 antibody

Catalog No. ABIN120312

The Mouse Monoclonal anti-2B4 antibody (Clone 2B4-69) (ABIN120312) specifically detects 2B4 in FACS and IP. The antibody is reactive with Human samples.

Quick Overview for 2B4 antibody (ABIN120312)

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This 2B4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 2B4-69

Product Details anti-2B4 Antibody

Specificity: This antibody is specific for the CD244 cell surface antigen, also known as natural killer receptor 2B4 (NKG2B4).

Purification: Affinity Chromatography on Protein G.

Immunogen: CD244 Fusion protein. Spleen cells from immunised Balb/c mice were fused with cells of the NS-1 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of 1/50-1/200 diluted antibody to label 106 cells in 100 μL. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for 2B4

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Alternative Name: CD244

Background: The glycoprotein CD244 is a member of the SLAM family of receptors which is expressed on natural killer (NK) cells, gamma delta T cells, CD14+ monocytes, and a subset of CD8+ T cells. CD244 was originally identified as an activating receptor on NK cells, although recent studies suggest that the receptor may have a wide range of activities including NK cell activation, costimulation, or even inhibition, depending on the cell type and stage of differentiation. The ligand of 2B4 is CD48, a GPI-anchored membrane protein also belonging to the SLAM family.Synonyms: NAIL, NK cell activation-inducing ligand, NK cell type I receptor protein 2B4, NKR2B4, Natural killer cell receptor 2B4

Gene ID: 51744

NCBI Accession: NP_001160135

UniProt: Q9BZW8