2B4 antibody (PE)

Catalog No. ABIN120314

The Mouse Monoclonal anti-2B4 antibody is suitable to detect 2B4 in samples from Human. It has been validated for FACS.

Quick Overview for 2B4 antibody (PE) (ABIN120314)

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This 2B4 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: 2B4-69

Product Details anti-2B4 Antibody (PE)

Specificity: This antibody is specific for the CD244 cell surface antigen, also known as natural killer receptor 2B4 (NKG2B4).

Purification: Affinity chromatography on Protein G

Immunogen: CD244 Fusion protein. Spleen cells from immunised Balb/c mice were fused with cells of the NS-1 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: neat - 1/10, use 10 μL of the suggested working dilution to label 106 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL distilled water

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Can be shipped at 2-8 °C. Upon arrival store (in aliquots) at -20 °C.

Target Details for 2B4

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Alternative Name: CD244

Background: The glycoprotein CD244 is a member of the SLAM family of receptors which is expressed on natural killer (NK) cells, gamma delta T cells, CD14+ monocytes, and a subset of CD8+ T cells. CD244 was originally identified as an activating receptor on NK cells, although recent studies suggest that the receptor may have a wide range of activities including NK cell activation, costimulation, or even inhibition, depending on the cell type and stage of differentiation. The ligand of 2B4 is CD48, a GPI-anchored membrane protein also belonging to the SLAM family.Synonyms: NAIL, NK cell activation-inducing ligand, NK cell type I receptor protein 2B4, NKR2B4, Natural killer cell receptor 2B4

Gene ID: 51744

NCBI Accession: NP_001160135

UniProt: Q9BZW8