LAG3 antibody (PE)

Catalog No. ABIN120325

This Rat Monoclonal antibody specifically detects LAG3 in FACS. It exhibits reactivity toward Mouse.

Quick Overview for LAG3 antibody (PE) (ABIN120325)

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This LAG3 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: C9B7W

Product Details anti-LAG3 Antibody (PE)

Specificity: This antibody recognises lymphocyte activation gene-3 (LAG-3), that is also referred to as CD223. Clone C9B7W recognises an epitope within the D2 domain of CD223. The antibody is reported to block the in vitro function of murine LAG-3 but studies suggest that the antibody does not block binding of LAG-3 to MHC class II. We recommend the use of SM2270LE for this purpose.

Purification: Affinity Chromatography on Protein G.

Immunogen: Murine CD223 Ig fusion protein. Cells from immunised Lewis rats were fused with cells of the Sp/20 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of Neat-1/5 diluted antibody to label 10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL distilled water

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Can be shipped at 2-8 °C. Upon arrival, aliquot and store (in aliquots) at -20 °C.

Target Details for LAG3

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Alternative Name: CD223 / LAG3

Background: CD223 is a 70kD activation-induced cell surface molecule. Murine CD223 is expressed on activated CD4 positive and CD8 positive alpha/beta T lymphocytes and a subset of natural killer (NK) cells. CD223 binds to MHC class II molecules with high affinity and is reported to negatively regulate T cell homeostasis and T cell expansion.Synonyms: FDC, LAG-3, Lymphocyte activation gene 3 protein

Gene ID: 16768

NCBI Accession: NP_032505

UniProt: Q61790

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints