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CD4 antibody (Domain 2)

The Mouse Monoclonal anti-CD4 antibody has been validated for FACS, IP and IHC (fro). It is suitable to detect CD4 in samples from Rat.
Catalog No. ABIN120380

Quick Overview for CD4 antibody (Domain 2) (ABIN120380)

Target

See all CD4 Antibodies
CD4

Reactivity

  • 399
  • 249
  • 133
  • 48
  • 30
  • 24
  • 20
  • 18
  • 17
  • 11
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  • 5
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  • 1
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Rat

Host

  • 416
  • 155
  • 152
  • 7
  • 3
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  • 1
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Mouse

Clonality

  • 589
  • 141
  • 5
Monoclonal

Conjugate

  • 227
  • 73
  • 63
  • 47
  • 42
  • 16
  • 12
  • 12
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  • 7
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  • 3
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  • 3
  • 3
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  • 2
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  • 2
This CD4 antibody is un-conjugated

Application

  • 611
  • 199
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  • 78
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  • 56
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  • 1
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  • 1
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone

OX-35
  • Binding Specificity

    • 27
    • 16
    • 16
    • 16
    • 16
    • 12
    • 11
    • 9
    • 9
    • 9
    • 8
    • 4
    • 3
    • 3
    • 2
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    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    Domain 2

    Purification

    Affinity chromatography on Protein G

    Immunogen

    MLR generated rat T helper lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line

    Isotype

    IgG2a
  • Application Notes

    Flow cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Immunoprecipitation.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    PBS, pH 7.4 containing 0.09 % Sodium Azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target

    CD4

    Alternative Name

    CD4

    Background

    CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

    Gene ID

    10116

    UniProt

    P05540

    Pathways

    TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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