CD4 antibody (Domain 2)
Quick Overview for CD4 antibody (Domain 2) (ABIN120380)
Target
See all CD4 AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Binding Specificity
- Domain 2
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Purification
- Affinity chromatography on Protein G
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Immunogen
- MLR generated rat T helper lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line
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Isotype
- IgG2a
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Application Notes
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Flow cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- PBS, pH 7.4 containing 0.09 % Sodium Azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid repeated freezing and thawing.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- CD4
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Alternative Name
- CD4
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Background
- CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
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Gene ID
- 10116
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UniProt
- P05540
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Pathways
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
Target
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