SIRPA antibody

Catalog No. ABIN120470

The Mouse Monoclonal anti-SIRPA antibody has been validated for WB, FACS, IHC (p) and IHC (fro). It is suitable to detect SIRPA in samples from Rat.

Quick Overview for SIRPA antibody (ABIN120470)

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIRPA antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: OX-41

Product Details anti-SIRPA Antibody

Specificity: This antibody recognises CD172a, also known as Signal regulatory protein (SIRP). MRC OX-41 binds to a different epitope on SIRP than ED9. It does not block the interaction of CD172a - CD47.

Purification: Affinity chromatography on Protein G

Immunogen: Rat peritoneal macrophages. Spleen cells from immunised BALB/c mice were fused with the NSO/U mouse myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Western Blot. Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen and paraffin sections: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SIRPA

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Alternative Name: CD172a / SIRPA

Background: CD172a is expressed selectively by myeloid cells and by neurons.Synonyms: BIT, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, MFR, MYD1, Macrophage fusion receptor, MyD-1 antigen, PTPNS1, SHP substrate 1, SHPS-1, SHPS1, Signal-regulatory protein alpha-1, Sirp-alpha-2, Sirp-alpha-3, Tyrosine-protein phosphatase non-receptor type substrate 1, p84

Gene ID: 25528

NCBI Accession: NP_037148

UniProt: P97710