Albumin antibody

Catalog No. ABIN120541

This Mouse Monoclonal antibody specifically detects Albumin in WB, EIA, RIA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Albumin antibody (ABIN120541)

Target: Albumin (ALB)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Albumin antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AL-01

Product Details anti-Albumin Antibody

Specificity: The antibody AL-01 reacts only with Human Serum Albumin, a 65-67 kDa monomeric protein in Human blood plasma, it is produced in liver. No cross-reactivity was observed with other serum proteins. Negative with Other Mammalian (except Human)

Purification: Precipitation Methods

Immunogen: Fraction of proteins containing albumin after ammonium sulphate precipitation and DEAE- chromatography of human serum

Isotype: IgG1

Application Details

Application Notes: ELISA. RIA. Western blotting. Immunohistochemistry (Paraffin): 5 m g/mL. Positive tissue: liver.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for Albumin

Target: Albumin (ALB)

Alternative Name: Albumin

Background: Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. Human serum albumin (65-67 kDa) is the most abundant protein in human blood plasma (produced in the liver). It has a serum half-life of approximately 20 days.Synonyms: ALB, BSA, HSA, Serum Albumin

Molecular Weight: 69 kDa

Gene ID: 213

UniProt: P02768

Pathways: Lipid Metabolism