GLB1 antibody

Catalog No. ABIN120543

This anti-GLB1 antibody is a Mouse Monoclonal antibody detecting GLB1 in WB and IF. Suitable for E. coli. This Primary Antibody has been cited in 3+ publications.

Quick Overview for GLB1 antibody (ABIN120543)

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Reactivity: E. coli

Host: Mouse

Clonality: Monoclonal

Conjugate: This GLB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: BG-02

Product Details anti-GLB1 Antibody

Specificity: The antibody recognizes a non-catalytic centre epitope of beta-Galactosidase from E. coli.

Purification: Precipitation Methods.

Immunogen: beta-Galactosidase purified from E. coli

Isotype: IgG1

Application Details

Application Notes: Immunocytochemistry. Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

References for anti-GLB1 antibody (ABIN120543)

Tahirovic, Hellal, Neukirchen, Hindges, Garvalov, Flynn, Stradal, Chrostek-Grashoff, Brakebusch, Bradke: "Rac1 regulates neuronal polarization through the WAVE complex." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 20, pp. 6930-43, (2010) (PubMed).

Dráber, Slavícková, Sládecek, Viklický: "Monoclonal antibodies to Escherichia coli beta-galactosidase and their use for detection and purification of recombinant expression products." in: Hybridoma, Vol. 11, Issue 3, pp. 385-90, (1992) (PubMed).

Bazil, Horejsí, Baudys, Kristofová, Strominger, Kostka, Hilgert: "Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen." in: European journal of immunology, Vol. 16, Issue 12, pp. 1583-9, (1987) (PubMed).

Target Details for GLB1

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Alternative Name: beta Galactosidase (GAL)

Background: Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.Synonyms: Beta-Gal Fusion Protein, Beta-Gal tag, JW0335, Lactase, b0344, lacZ tag

Gene ID: 945006

UniProt: P00722

Pathways: Glycosaminoglycan Metabolic Process