MME antibody

Catalog No. ABIN120550

This Mouse Monoclonal antibody specifically detects MME in WB, FACS and IP. It exhibits reactivity toward Human.

Quick Overview for MME antibody (ABIN120550)

Target: MME (Membrane Metallo-Endopeptidase (MME))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MME antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: MEM-78

Product Details anti-MME Antibody

Specificity: The antibody reacts with CD10 antigen (CALLA - Common acute lymphatic leukemia antigen), a 100 kDa type II integral membrane protein.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Protein A affinity chromatography

Immunogen: NALM-6 human pre-B cell line

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: use 5 μL of neat antibody to label 10^6 cells. Immunoprecipitation. Western Blot (non-reducing conditions).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for MME

Target: MME (Membrane Metallo-Endopeptidase (MME))

Alternative Name: CD10 / Neprilysin

Target Type: Chemical

Background: CD10 (neutral endopeptidase NEP, common acute lymphocytic leukemia antigen CALLA, membrane metallo-endopeptidase MME, enkefalinase) is a 100- kDa cell surface zinc metalloprotease cleaving peptide bonds on the N-terminus of hydrophobic amino acids and inactivating multiple physiologically active peptids. CD10 is expressed on various normal cell types, including lymphoid precursor cells, germinal center B lymhocytes, and some epithelial cells, and its expression level serves as a marker for diagnostics of many carcinomas. CD10 is also a differentiation antigen for early B-lymphoid progenitors in the B-cell differentiation pathway and has a key role in regulation of growth, differentiation and signal transduction of many cellular systems.Synonyms: Atriopeptidase, CALLA, Common acute lymphocytic leukemia antigen, EPN, Enkephalinase, MME, NEP, Neutral endopeptidase, Neutral endopeptidase 24.11

Gene ID: 4311

UniProt: P08473

Pathways: RTK Signaling, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration