CD99 antibody (Isoform CD99R)

Catalog No. ABIN120623

The Mouse Monoclonal anti-CD99 antibody has been validated for FACS, WB and IP. It is suitable to detect CD99 in samples from Human.

Quick Overview for CD99 antibody (Isoform CD99R) (ABIN120623)

Target: CD99

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD99 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)

Clone: MEM-131

Product Details anti-CD99 Antibody

Binding Specificity: Isoform CD99R

Specificity: The antibody reacts with CD99R, an epitope restricted to a subset of CD99 Molecule expressed on myeloid cells, NK cells and T lymphocytes.

Purification: Precipitation methods

Purity: > 95 % (by SDS-PAGE)

Immunogen: HPB-ALL human peripheral blood leukemia T-cell line

Isotype: IgM

Application Details

Application Notes: Flow Cytometry. Immunoprecipitation. Western Blotting under non reducing conditions. Immunohistochemistry (paraffin sections): Recommended dilution: 10 m g/mL, Positive tissue: thymic lymphocytes
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody at 2 - 8 °C. Do not freeze.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for CD99

Target: CD99

Alternative Name: CD99 / MIC2

Background: CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.Synonyms: 12E7, E2 antigen, MIC2X, MIC2Y, Protein MIC2, T-cell surface glycoprotein E2

Gene ID: 4267

UniProt: P14209