Cytokeratin 18 antibody

Catalog No. ABIN120625

This Mouse Monoclonal antibody specifically detects Cytokeratin 18 in WB, IF, FACS, IHC (p), IP and EIA. It exhibits reactivity toward Mammalian.

Quick Overview for Cytokeratin 18 antibody (ABIN120625)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivity: Mammalian

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 18 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: C-04

Product Details anti-Cytokeratin 18 Antibody

Specificity: The antibody C-04 reacts with Cytokeratin 18 (45 kDa).

Purification: Affinity Chromatography on Protein A

Immunogen: Cytoskeleton preparation of epidermal carcinoma cell line A431

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on paraffin sections. Flow Cytometry. Immunoprecipitation. Immunocytochemistry. ELISA: The antibody C-04 has been tested as the capture antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with antibody DA-7 (cat. no. 1B-110-C100) and with the antibody DC-10 (cat. no. 1B-107-C100). Western Blot: 1-2 mg/mL, overnight in 4 °C, Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer, Non-reducing conditions. SDS-PAGE (10 % separating gel). Positive control: HeLa human cervix carcinoma cell line, MCF-7 human breast adenocarcinoma cell line.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4 with 15 mM Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Alternative Name: Cytokeratin 18

Background: Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratins 18 belongs to type I family (acidic cytokeratins).Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18

Gene ID: 3875

UniProt: P05783

Pathways: Apoptosis, Caspase Cascade in Apoptosis